Media prep
Confirms recipe, material status, conductivity, pH, and hold conditions.
Upstream control
Hold the culture in its narrow viability window.
Bioseon controls media prep, seed train, and production bioreactors across pH, dissolved oxygen, temperature, agitation, CO₂, and fed-batch or perfusion feed while watching VCD and titer.
pH · DO · feed
Closed-loop cell culture.
Designed for mAbs, vaccines, GLP-1 and recombinant proteins, and cell and gene therapy manufacturing.
The upstream problem.
Cells grow product only when biology, chemistry, and fluid dynamics stay aligned. Feed or gas drift can reduce viability, slow VCD growth, shift titer, or create CQA risk before standard sampling catches it.
- Narrow viability window over days or weeks
- Delayed lab measurements for titer and quality
- Manual feed and gas adjustments under time pressure
Control risk signals
Bioseon fuses DCS tags, PAT and Raman signals, lab data, historian context, and environmental observations into one live view of culture health.
Media and seed control.
The media-and-seed agent tracks media and buffer prep, single-use assemblies, seed-train phase, inoculum readiness, and contamination risk before production starts.
- Composition checks against recipe and lot context
- Seed train growth state and transfer timing
- Readiness handoff to production bioreactor control
Seed train
Monitors expansion, viability, transfer criteria, and contamination guardrails.
Culture and feed control.
The culture-and-feed agent adjusts setpoints inside validated envelopes, using soft sensors and the twin to keep growth and productivity aligned with the target batch trajectory.
pH
Maintains acid and base correction around the cell-line window.
DO
Coordinates oxygen, gas mix, sparge, and agitation response.
Temperature
Tracks shifts for growth, production, and quality programs.
Agitation
Balances mixing, mass transfer, and shear sensitivity.
CO₂
Controls gas strategy against pH and culture state.
Feed
Optimizes bolus, fed-batch, or perfusion feed against VCD and titer.
Soft-sensing titer and VCD.
Bioseon estimates viable cell density, titer, and culture trajectory from PAT, Raman, process variables, and lab references. Operators see where the batch is heading before the next offline result.
BR-04 live state
- pH 7.02, DO 41 percent, temperature 36.9 C
- VCD 18.4e6 cells per mL, titer 6.41 g/L
- Actions held inside validated control envelopes
Fed-batch and perfusion control.
| Mode | Control focus | Bioseon action |
|---|---|---|
| Fed-batch | Bolus and continuous feeds, metabolite balance, growth and production phases | Adaptive feed timing with pH, DO, temperature, agitation, and CO₂ coordination |
| Perfusion | Cell retention, media exchange, bleed strategy, steady productivity | Perfusion setpoint control against VCD, titer, and CQA risk |
| Hybrid processes | Scale-up and tech transfer across lab, pilot, and production | Twin-guided trajectories from historical and live batch behavior |
From media prep to harvest.
- 01
Media prep
Verify materials, recipe, pH, conductivity, sterility, and hold state.
- 02
Seed train
Expand cells to inoculum target with viability and contamination checks.
- 03
Inoculation
Confirm vessel readiness, transfer timing, and initial control state.
- 04
Exponential growth
Maintain pH, DO, temperature, agitation, CO₂, and feed around VCD growth.
- 05
Production
Optimize productivity while watching titer and CQA risk.
- 06
Harvest
Hand off to clarification and downstream purification at the right culture state.
Upstream outcomes.
VCD
Stabilized. Growth is held closer to the intended trajectory.
TITER
Increased. Feed and production phases adapt to live culture state.
DEVIATIONS
Reduced. Drift is caught earlier with explainable actions.
MEDIA
Optimized. Feed and single-use inputs are used against measured need.
Contamination guardrails around the culture.
Bioseon combines bioburden, environmental monitoring, material status, process drift, and operator context to flag contamination and deviation risk while there is still time to respond.
- Environmental and process signal fusion
- Seed train and media-prep risk context
- GMP deviation record linked to batch phase
Related capabilities.
Titer and CQA sensing
Soft sensors for titer, VCD, metabolites, and quality signals.
Explore sensingDigital twin
Predict culture trajectory and choose control actions before drift becomes deviation.
Explore twinDownstream
Carry the target batch state into harvest, capture, polish, UF/DF, and viral steps.
Explore downstreamPricing for upstream lines.
Start on one production bioreactor at $13,000 per bioreactor or skid per month, expand to Site at $85,000 per month, or plan Enterprise land from $700k–$8M ACV.
Best first line
Choose a high-value production bioreactor with enough historical batches to calibrate soft sensors and compare shadow-mode recommendations.
Upstream questions.
Which variables can Bioseon control?
Bioseon controls pH, dissolved oxygen, temperature, agitation, CO₂, and fed-batch or perfusion feed inside validated process envelopes.
Does it work with perfusion?
Yes. Bioseon supports single-use and perfusion environments, including cell-retention and media-exchange strategies tied to VCD, titer, and CQA risk.
How are titer and VCD watched in real time?
Soft sensors combine PAT, Raman, DCS tags, historian data, and lab references to estimate live state between offline measurements.
What happens before closed-loop control?
Bioseon runs in shadow mode, compares recommendations to operator actions, validates model accuracy, and then enables constrained action with human override.
Give your culture a live control layer.
Scope Bioseon on one upstream line and quantify VCD, titer, deviation, and media impact.